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Rapid tests woman health


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Prelabour rupture of membranes PROM is usually diagnosed by physical examination; but in cases ambiguous could be necessary the use  commercialkits  to confirm the diagnosis. Given the significance of the diagnosis of PROM  that may lead to treatments that could be aggressive, the SEGO (Spanish society of gynaecology and obstetrics)  recommends asure  the  diagnosis, among other methods, through the implementation of rapid tests for the detection of biomarkers in vaginal fluids. So begins the treatment quickly only to the necessary case.
These kits are based on the determination of specific biomarkers that are found in high quantities in the amniotic fluid in comparison with those found in the vaginal fluid. Many studies have shown the superiority of the new generation of these test non-invasive which are very easy to use, to classic methods that are practically obsolete due to its lower reliability and precision.
Currently two biomarkers for the  PROM in vitro diagnostic with  greater sensitivity and specificity are:
1 IGFBP-1. (Insulin-like growth factor binding protein - 1) Carrying the insulinosimil growth factor protein. This protein is absent in vagina, so its presence is significant demembrans rupture.
2 PAMG-1 placental alpha microglobulin-1. This protein is found in very low levels in blood and extremely low in cervicovaginal secretions when chorioamniotic membranes are intact
A study about 1385 women, showed that statistically significant differences there are no when comparing the two tests in the same clinical conditions. IGFBP-1 is the only one who has proven to have the presence of maternal in the sample blood, resistance front while PAMG_1 can cause false positive results. False negatives are rare but are given when the test passes more than twelve hours since it is believed that the bag has been broken (after more 12 hours is recommended for another type of studies since vaginal proteases can degrade the IGFBP_1.)
The IGFBP-1  test consists of a test Immunochromatographic very easy to perform. Individual kit contains everything for the test.
· A cotton swab that is inserted into the vagina for the extraction of the sample.
· A vial containing the solvent to extract the protein from the swab.
· A test strip which is inserted into the vial and used to make the reading.
The reading of the result is simple: two lines: test positive, a-line: negative test. All kits have a control line to check that the kit is in good condition.
Performing the test is  very easy :  insert the swab in the vaigna, take it for about a minute into the vial of buffer and then read the Strip within 10 minutes.

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